Unit · year 3
BU-306 · Structural Biology & Biophysics
Threads structure · energy25 lectures5 concepts
The physical principles behind macromolecular structure and function.
PREREQUISITES
Lectures
| L01 | Structure Determines Function: The Biophysical View — |
| L02 | Forces Stabilising Macromolecules |
| L03 | The Protein Folding Problem |
| L04 | Anfinsen's Experiment |
| L05 | The Folding Funnel and Energy Landscape |
| L06 | Chaperones and Assisted Folding |
| L07 | Misfolding and Disease |
| L08 | X-Ray Crystallography: Principles |
| L09 | Crystallisation and Diffraction |
| L10 | The Phase Problem and Structure Solution |
| L11 | Resolution and Model Quality |
| L12 | Cryo-EM and NMR as Alternatives |
| L13 | Enzyme Kinetics: Rate and Substrate |
| L14 | The Michaelis–Menten Equation |
| L15 | KM, Vmax, and Catalytic Efficiency |
| L16 | Enzyme Inhibition and Its Kinetic Signatures |
| L17 | Cooperativity and the Hill Equation |
| L18 | Allosteric Regulation |
| L19 | The MWC and KNF Models |
| L20 | Feedback Inhibition in Pathways |
| L21 | Molecular Motors: The Class |
| L22 | Myosin, Kinesin, and Dynein |
| L23 | Mechanochemical Coupling and Step Size |
| L24 | Single-Molecule Methods |
| L25 | Synthesis: Machines Built from Molecules |
Concepts in this unit
T-092
Protein folding
The thermodynamics that select the native state.
T-093
X-ray crystallography
Determining structure from diffraction.
T-094
Enzyme kinetics
The Michaelis-Menten model of catalysis.
T-095
Allosteric regulation
Control by binding at a site away from the active site.
T-096
Molecular motors
Proteins that convert chemical energy into motion.